Valley Fever Virus by Enzyme-Linked Immunosorbent Assay
نویسندگان
چکیده
Rift Valley fever virus (RVFV) is an important human and animal pathogen in Africa and has been responsible for infections in travelers. Because of the aerosol infectivity and risk of dissemination of the virus, a need exists for simple, safe, serological tests for diagnosis. An enzyme-linked immunosorbent assay (ELISA) was developed to detect RVFV-specific immunoglobulin (immunoglobulin G [IgG] and IgM). In the test, a betapropiolactone-inactivated, sucrose-acetone-extracted, suckling mouse liver RVFV antigen
منابع مشابه
The use of su.crose-acetone-extracted Rift Valley fever virus antigen derived from cell culture in an indirect enzyme-linked immunosorbent assay and haemagglutination-inhibition
PAWESKA, J.T. , BARNARD, B.J.H. & WILLIAMS, R. 1995. The use of sucrose-acetone-extracted Rift Valley fever virus antigen derived from cell culture in an indirect enzyme-linked immunosorbent assay and haemagglutination-inhibition test. Onderstepoort Journal of Veterinary Research, 62:227-233 A sucrose-acetone-extracted , Madin-Darby-bovine-kidney (MDBK)-derived Rift Valley fever virus (RVFV) an...
متن کاملDetection of Rift Valley fever virus antigen by enzyme-linked immunosorbent assay.
A double-antibody (sandwich) enzyme-linked immunosorbent assay (ELISA) was adapted to detect Rift Valley fever virus antigen. Antibodies were purified from hyperimmune mouse and rabbit sera by affinity chromatography, using CNBr-activated Sepharose 4B coupled to a beta-propiolactone-inactivated sucrose-acetone-extracted suckling mouse liver antigen. In the assay, antigen was captured by mouse a...
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A retrospective study was undertaken to examine domestic ruminant sera for Rift Valley fever (RVF) virus antibodies. The sera were collected between 1992 and 1995 from cattle, sheep and goats from various locations in Saudi Arabia. The standard capture enzyme-linked immunosorbent assay system was employed to detect specific RVF antibodies in the animals and the results indicated an absence of R...
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Due to the unpredictable and explosive nature of Rift Valley fever (RVF) outbreaks, rapid and accurate diagnostic assays for low-resource settings are urgently needed. To improve existing diagnostic assays, monoclonal antibodies (MAbs) specific for the nucleocapsid protein of RVF virus (RVFV) were produced and characterized. Four IgG2a MAbs showed specific binding to denatured nucleocapsid prot...
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تاریخ انتشار 2003